MITEs (Miniature Inverted-repeat Transposable Elements) are AT rich short repeat sequences, typically 70–800 bp in length exhibiting the structural features of DNA transposons, containing terminal inverted repeats (TIRs) flanked by small direct repeats (target site duplication, TSD) at both the ends of the element. Apart from playing crucial roles in gene regulation and genome evolution, MITE-related sequences could also encode small RNAs, which regulate specific target genes at the transcriptional and post-transcriptional levels.
Chromosome level MITEs were identified in TV1 genome by MITE Tracker. A total 22219 MITEs were identified clustered in 1913 families with sequence length ranging from 4 to 798 nucleotide bases. Moreover the MITES were classified as genic, near genic and intergenic based upon their locations within or outside the gene sequences. The genic region is defined as a region where MITEs found within a gene, near genic region consists of sequences within a 1000-bp sequences upstream or downstream to a gene, whereas intergenic MITEs were located beyond the gene and its 1000 bp flanking regions.